QPCR Real Time PCR Assay | Assay Design

MOgene utilizes the Applied Biosystems® 7900HT from Thermo Scientific®

Real Time PCR

Real-time PCR remains one of the most sensitive and quantitative tools for gene expression used today. MOgene offers both custom and pre-optimized QPCR assays for an assortment of specific applications. We manage all aspects of the project from assay design, to nucleic acid isolation, to data analysis.

High-throughput Meets Customization

Utilizing ABI’s 7900HT and TaqMan® 384-well micro fluidic cards MOgene has the capability of running hundreds of real-time PCR reactions simultaneously. We require only small amounts of sample, to run 1 to 8 samples in parallel against 12 to 384 TaqMan® Gene Expression Assay targets.

Service Features and Options

qPCR primer and probe design
Nucleic acid isolation from tissue, cells, biotechnology products, etc.
Quantitation using a NanoDrop® ND-1000
Qualitative analysis by an Agilent BioAnalyzer®
Primer/Probe optimization
Samples assayed in duplicate to ensure reproducibility
Controls included on each thermocycling run
Data report with project design description
Statistical analysis upon request

Request a Quote

At MOgene, we connect with our customers to make sure we understand the scope and requirements of each project, and to ensure that our data arrives quickly to advance your science. Start by filling out our quick Quote Request, and we’ll contact you promptly to work through the technical and delivery details for your project.

Testimonials

I have been involved with microarrays for almost a decade—starting with homemade cDNA arrays for one of our cyanobacterial strains. This was a very fruitful enterprise, but the advent of the Agilent arrays provides speed and flexibility. It was not easy to make the change, in part because of our previous success with the old system, and, in part, because of the ability to do everything ourselves and to train students and postdocs. Well, MOgene has helped us out in all regards! We have now successfully used MOgene with the Agilent arrays for two cyanobacteria and your work has been excellent.

Even better, I appreciate the way you hosted a graduate student so that she could learn all of the details of microarray analysis—from hybridization to microarray reading to initial analysis. This total service was greatly appreciated and settled any argument about how to proceed in the future—we will continue to order Agilent arrays and work with MOgene to achieve high quality results! Thanks for all of your help.

Jared Ordway, PhD Director of Research and Development Orion Genomics, LLC