Real Time PCR

Real-time PCR remains one of the most sensitive and quantitative tools for gene expression used today. MOgene offers both custom and pre-optimized QPCR assays for a wide variety of applications such as gene (mRNA, microRNA and non-coding RNA) expression, genetic variation (SNP genotyping, DME genotyping or Copy Number Variation) analysis, mutation detection analysis, and epigenetic analysis in “RUO” or “regulated” projects. We manage all aspects of the project from assay design, to nucleic acid isolation, to data analysis.

High-throughput Meets Customization

Utilizing ABI’s QuantStudio 12k Flex Real-Time PCR system MOgene has the capability of running hundreds of real-time PCR reactions simultaneously. This flexible system allows us to provide you a collection of different configurations to match your requirements.

Service Features and Options

• qPCR primer and probe design
• Nucleic acid isolation from tissue, cells, biotechnology products, etc.
• Quantitation using a NanoDrop® ND-1000
• Qualitative analysis by an Agilent BioAnalyzer®
• Primer/Probe optimization
• Samples assayed in duplicate to ensure reproducibility
• Controls included on each thermocycling run
• Data report with project design description
• Statistical analysis upon request

Request a Quote

At MOgene, we connect with our customers to make sure we understand the scope and requirements of each project, and to ensure that our data arrives quickly to advance your science. Start by filling out our quick Quote Request, and we’ll contact you promptly to work through the technical and delivery details for your project.