Pacific Biosciences Sequencing

MOgene now offers all sequencing services on the Pacific Biosciences Sequel sequencing system. The Sequel system, a next generation of SMRT (Single-molecule, real-time) sequencing system, offers much higher throughput and more scalability than the second-generation sequencing (SGS) technologies, making it well-suited for unsolved problems in genome, transcriptome, and epigenetics research.

The highly-contiguous de novo assemblies using PacBio sequencing can close gaps in current reference assemblies and characterize structural variation (SV) in personal genomes. With longer reads, we can sequence through extended repetitive regions and detect mutations, many of which are associated with diseases. Moreover, PacBio transcriptome sequencing is advantageous for the identification of gene isoforms and facilitates reliable discoveries of novel genes and novel isoforms of annotated genes, due to its ability to sequence full-length transcripts or fragments with significant lengths.

Additionally, PacBio’s sequencing technique provides information that is useful for the direct detection of base modifications, such as methylation. In addition to using PacBio sequencing alone, many hybrid sequencing strategies, such as combination of PacBio sequencing and BioNano optical mapping data, have been developed to make use of more accurate short reads in conjunction with PacBio long reads. In general, hybrid sequencing strategies are more affordable and scalable especially for small-size laboratories than using PacBio Sequencing alone. The advent of PacBio sequencing has made available much information that could not be obtained via SGS alone.

Applications include:

  • De Novo Assembly
  • Exomes and Whole Genomes
  • Targeted Sequencing
  • Base Modifications
  • Isoform Sequencing Detection
  • Amplicon\COSMID Sequencing

Areas include:

  • Human
  • Plant & Animal
  • Microbiology

PacBio Sample Submission Guidelines
Please review following documents for DNA or RNA (IsoSeq) sample submission and shipping guidelines.
Sample Guidelines\Pacbio Guidelines – DNA v2.0.pdf
Sample Guidelines\Pacbio Guidelines – Iso Seq v2.0.pdf
Sample Guidelines\Pacbio Guidelines – DNA Shipping Handling v2.0.pdf