Technology Overview

Generating a BioNano map begins with the labeling long, megabase size DNA molecules at specific sequence motifs to generate a unique barcode like pattern. The labeled DNA is processed using NanoChannel array technology that unravels, sorts, and confines native-state genomic DNA fragments in a linearized conformation. Multiple DNA molecules are optically imaged and digitized to create optical maps. These long molecules spanning beyond a field of view are stitched together and provide genetic information about molecular organization. Inconsistent features can reflect sites of DNA damage, translocations, tandem repeats, duplications and deletions, epigenetic markers, or other functional biological events.

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Applications

            Next Generation Mapping:

Next-generation mapping (NGM) by the Irys® System, the next great evolution in genomics, reveals the long range information that enables mapping physical genome at much higher resolution in a cost effective manner in a matter of days.

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           Structural Variation Analysis:

BioNano maps can be used for the analysis of large structural variation (SV) events greater than 1kbp.  Retaining long-range contiguity throughout the genome mapping process is critical for any comprehensive study of genome structure and function, in particular de novo sequence scaffolding and analysis of structural variation in complex genomes. Structural variants and repeats are measured directly within long, single-molecule “reads” for comprehensive analysis of what has been dubbed “the inaccessible genome.”

 

BNG SV Graphic

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            Hybrid Scaffolding:

BioNano maps provide dense genome-wide anchor points for ordering and orienting sequencing contigs or scaffolds to significantly increase the completion and accuracy of de novo assemblies.

 

Hybrid Scaffold Example

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Contact us to learn more about getting a BioNano map or go to http://www.bionanogenomics.com