| Arrays: We offer high content, Agilent pre-printed, catalog microarrays for gene expression analysis–across a wide range of genomes. If a commercial array is not available for your specific needs, or organism, we have considerable experience with custom design of microarrays. We have designed arrays for both prokaryote and eukaryote species.
RNA QA: We perform rigorous RNA sample quality assessment using the Agilent 2100 bioanalyzer and RNA LabChip Kits.
Labeling: We label cRNA or cDNA samples using Agilent’s labeling kits that have been optimized for Agilent DNA microarrays. MOgene has also developed high-efficiency labeling protocols for microbial RNA (i.e. with no polyA tails). We determine labeling efficiency using Agilent’s special set of protocols to maximize dynamic range.
Scanning: We scan all microarrays on Agilent’s Microarray Scanner with SureScan technology that provides added sensitivity and performance.
Feature Extraction: Feature extraction of all microarrays is performed using Agilent’s special software that reduces false positive and false negative results through unique background correction algorithms.
Data QA: We perform QC on each array to ensure that the data we provide is of high quality. We check for consistent background, mean signal and differential expression values.
Analysis: We can analyze microarray results with confidence using the Rosetta LuminatorTM gene expression data analysis system.
Sample tracking: To maintain confidence, reliability and confidentiality we use barcode tracking throughout the process: from RNA samples to microarray slides to final feature extracted spots and QC steps to analyzed results. |
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Experimental design consultation: A key step for obtaining interpretable data begins with careful experimental design. We offer consulting services to help you get the most biological insight out of your data.
Custom design of microarrays: No commercial arrays available for your model organism? Let us design an array for you!
Data Analysis: So you can focus on the science, bioinformatics, statistics, and custom analysis services for the large data set are also available:
- Custom data formats and reports to aide interpretation of the data
- Statistical analysis of fluor-reverse, biological and process replicates to minimize false-positives
- Biomarker discovery
- Identification of functions and processes affected by perturbation (e.g. treatment or mutation). Inference of gene regulatory networks
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Send us the sample and we'll send you high quality data. |
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