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ImmunoSEQ Assay Services

To provide researchers insights into the adaptive immune system, MOgene offers immunoSEQ™ assays from Adaptive Biotechnologies. These let you explore broad characteristics of T-and B-cell repertoires, as well as the unique details of individual clones. Whether you are investigating the general immune trends or specific clones, we can provide you with reliable, quantitative and reproducible results through the immunoSEQ platform. Combining PCR amplification bias control with MOgene’s high throughput sequencing resources, we can process immunoSEQ Assays that let you identify millions of T- and B-cell receptors in a single sample.

Research experiments made possible by ImmunoSEQ include case-control, time series, and tissue vs. periphery assays with a broad range of assessments including clone tracking, repertoire profiling and identification of public clones using the immunoSEQ Assay.

 

Applications

Public Clones: Determine the shared receptor amino acid sequences. Sequencing data is delivered through the immunoSEQ Analyzer, a secure cloud-based platform of bioinformatics tools that make it easy to get to the most relevant aspects of your data and create publication-ready analysis images of your discoveries. You can even set up collaborations by sharing data with individuals, groups, or as public data sets from within your Analyzer account.

hsTCRB Assay: Generate quantitative measurements of the human T-cell receptor repertoire. Compatible sample sources are genomic DNA (gDNA) derived from Peripheral blood mononuclear cells (PBMCs), whole blood and tissue samples.

 

Additional research applications include:

  • Clone tracking: Track T-cell and B-cell receptors over time or between sample types
  • Monitor immune reconstitution after stem cell transplantation
  • Repertoire properties: Identify highly expanded clones and diversity of lymphocyte populations
  • Evaluate clonal and resident T-cells after skin immunization
  • Track glioma infiltrating T-cells after DC vaccination
  • Monitoring minimal residual disease by sequencing T-cell receptors in cutaneous T-cell lymphoma
  • Assessing CMV reactivation impact, as post-transplant T-cells reconstitute and result in defects of the underlying TCRB repertoire

 

Q&A

How quantitative is the immunoSEQ Assay? How do you address multiplex PCR bias?
Adaptive Biotechnologies has spent many years developing the immunoSEQ Assay to deliver high-quality, quantitative analysis of the immune repertoire, including a pioneering solution for bias control. Since amplification bias is a potential limitation of multiplex PCR, immunoSEQ uses synthetic immune cell receptor genes as controls for bioinformatic normalization of amplification bias for every V&J gene segment in the genome. This control is applied in-solution for every individual sample analyzed by the assay.

How are results form the immunoSEQ Assay provided?
Sequencing data is made available through the immunoSEQ Analyzer. The immunoSEQ Analyzer is a powerful online software suite that allows the viewing, analysis, and comparison of immunosequencing data with just a few simple clicks. The immunoSEQ Analyzer also readily enables the customization and download of high-quality analysis images suitable for presentation or publication.

Can I customize and export graphs from the immunoSEQ Analyzer for publication?
Yes. Most graphs and charts can be customized in terms of axes, labels, and colors. You can also export a high quality image file of the graph and further customize in your choice of software.

Can I export immunoSEQ data from the Analyzer?
Yes, sequencing data can be exported for use in other analytics programs. immunoSEQ’s R-Connector makes it easy to work directly with immunoSEQ data in R. It is an installable R package that simplifies the loading of immunoSEQ sequence data and metadata into an R environment.

Are there any publicly available controls or example data I can look at?
Yes. Published Projects are open-access interactive data sets, often associated with published articles. These data sets are created by the researchers and/or Adaptive Biotechnologies. After logging into the Analyzer website, visit the Published Projects area to learn more, explore and use these open data sets. See the Published Projects within the Analyzer help topic for more information.

Request a Quote

At MOgene, we connect with our customers to make sure we understand the scope and requirements of each project, and to ensure that our data arrives quickly to advance your science. Start by filling out our quick Quote Request, and we’ll contact you promptly to work through the technical and delivery details for your project.