Real-time PCR remains one of the most sensitive and quantitative tools for gene expression used today. MOgene offers both custom and pre-optimized QPCR assays for an assortment of specific applications. We manage all aspects of the project from assay design, to nucleic acid isolation, to data analysis.

High-throughput Meets Customization

Utilizing ABI’s 7900HT and TaqMan® 384-well micro fluidic cards MOgene has the capability of running hundreds of real-time PCR reactions simultaneously. We require only small amounts of sample, to run 1 to 8 samples in parallel against 12 to 384 TaqMan® Gene Expression Assay targets.

Service Features and Options

• QPCR primer and probe design
• Nucleic acid isolation from tissue, cells, biotechnology products, etc.
• Quantitation using a NanoDrop® ND-1000
• Qualitative analysis by an Agilent BioAnalyzer®
• Primer/Probe optimization
• Samples assayed in duplicate to ensure reproducibility
• Controls included on each thermocycling run
• Data report with project design description
• Statistical analysis upon request