MOgene, LC is now a Service Provider for all of the Nimblegen Products

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Gene Expression CGH Chip on chip DNA Methylation
Gene Expression

Roche NimbleGen provides a unique combination of high-density arrays, long-oligo probes, and flexible design capability that together provide superior results for advanced gene expression analysis. NimbleGen microarrays enable accurate, sensitive, and specific interrogation of genome-wide expression for any sequenced and annotated genome. Researchers also can tile through a genome at any desired spacing to discover genome-wide expression activity in both coding and noncoding regions and map transcripts with unparalleled accuracy. NimbleGen microarrays and services for both prokaryotic and eukaryotic genomes are the gene expression platform of choice as evidenced by a growing list of scientific publications.

CGH

Comparative Genomic Hybridization (CGH) measures DNA copy number differences between a test and reference genome. NimbleGen offers two types of CGH array products: whole-genome and custom targeted arrays. Whole-genome arrays measure DNA copy number gains and losses across entire genomes, while custom targeted arrays can interrogate a single chromosomal region or multiple loci of interest. In addition, custom designs can be created with either uniform or mixed-density probe spacing.

ChIP-on-Chip

Roche NimbleGen provides the most sensitive ChIP-chip (chromatin immunoprecipitation on chip) microarrays and services on the market. These arrays enable rapid identification of precise binding sites of specific DNA-binding proteins—such as transcription factors, histones, and polymerases—within a target genome, as well as uncover chromatin remodeling in any region of a genome. NimbleGen ChIP-chip microarrays and services have become the platform of choice supported by an ever-growing list of peer-reviewed publications.

DNA Methylation

Roche NimbleGen provides the most sensitive and specific DNA methylation microarrays and services on the market. Affinity-based methods for enrichment of methylated DNA fragments, such as MeDIP (Methylated DNA immunoprecipitation) or the MBD (Methyl Binding Domain Protein) methods, are the preferred sample preparation methods for identifying methylated DNA on NimbleGen arrays. Coupled with one of these enrichment methods, our arrays and services offer:

  • Precise identification of methylated DNA regions across whole genomes or within biologically focused regions including promoters and CpG islands.
  • Highly sensitive detection of methylated CpGs due to use of long, isothermal (50-75mer) oligonucleotide probes.
  • Comprehensive catalog collection of whole-genome tiling arrays and targeted Deluxe Promoter arrays for multiple organisms.