MOgene, LC is an Agilent Certified Facility and offers expert gene expression, miRNA, CGH and ChIP-on-Chip services. You send us the RNA or DNA and we will provide you with high quality data in a timely basis at a reasonable price.

We utilize state-of-the-art equipment to perform quality check on each step of the process to assure you get the best dynamic range and highest sensitivity. We have Nanodrop-1000 and Agilent Bioanalyzer-2100 for determining quality of RNA and measuring the specific activity of the labeled samples.


Reagents Sure Hyb Chambers 60-mer microarrays Nanodrop Scanner Analysis Gene Expression & CGH Bioanalyzer
Reagents

Agilent's Low RNA Input Fluorescent Linear Amplification Kit, based on a simple, patented procedure, is designed to amplify and label either total RNA or poly A(+) RNA to generate micrograms of cRNA (antisense) or cDNA (sense).


SureHyb Chambers

Agilent's new microarray hybridization chambers and accessories have been specially designed to deliver more robust, more reproducible results enabling better detection of genes …in an easy to use, trusted format. The result is a solution to hybridization that leverages a unique mixing bubble to facilitate efficient mixing during the 17-hour incubation period to produce more meaningful microarray results.

Features

  • Improved ease-of-use compared to the "legacy" 6-screw chamber-can now be assembled and disassembled without tools, in seconds rather than minutes
  • Enhanced quality of data, delivered through reduced signal CV% and log ratio variability
  • Higher sensitivity through increased feature signal strength with low background noise
  • Reduced microarray variability (Microarray-to-microarray median CV = 14.5% legacy 6-screw model vs. 7.4% with the new G2534A model)
  • Complete, economical solution based on Agilent's SureHyb platform (chamber assemblies, disposable gasket Slides, hybridization reagents)
Nanodrop-1000 Spectrophotometer
  • Full-spectrum UV-Vis absorbance analyses (220-750nm)
  • For measuring absorbance of DNA, RNA, dyes, proteins and microbial cell culture OD
  • 1 ul of sample
  • Large dynamic range: 2-3700 ng/ul of dsDNA
Analysis Gene Expression & CGH

The Agilent CGH Analytics software provides an intuitive user interface for visually exploring, detecting and analyzing aberration patterns from multiple Comparative Genomic Hybridization (CGH) microarray profiles. It accepts data output from Agilent Feature Extraction software and displays chromosomal deletions and amplifications at multiple zoom levels simultaneously. Take advantage of two different high speed statistical aberration detection algorithms to assist in the detection and mapping of aberration regions with high confidence.

60-mer microarrays

Agilent-designed 60-mer microarrays are processed through a powerful combination of probe design algorithms coupled with Agilent's probe selection process. This design method gives the customer maximum confidence in the probe design and prevents redundancy in gene coverage, empirically validated using 10 different tissues then, using Agilent's SurePrint technology, printed with a maskless & highly-flexible microarray printing based on industrial-scale inkjet technologies, and unique QC processes. The result is affordable, accessible pre-printed microarrays that content researchers can trust

Agilents Scanner

The Agilent DNA microarray scanner is a 48-slide scanning system that can read any mix of 1" x 3" glass slide microarrays (both Agilent and non-Agilent) and seamlessly feature extract them all using Agilent's Feature Extraction Software.

Built on a strong engineering foundation and years of experience in the microarray scanner market, the Agilent unleashes an enhanced-feature, powerful, fully-automated scanner with walk-away freedom.

Features

  • Uniform Images & Enhanced Sensitivity - A component of Agilent's SureScan technology - Dynamic autofocus continually adjusts to keep features in focus while scanning. This minimizes the effects of gradients and aberrations associated with glass slides, and results in improved uniformity and enhanced sensitivity.
  • Low Level Detection - Optimized precision optics, broad dynamic range and minimal spectral cross talk allow users to detect weak features and achieve rapid, accurate and dependable results.
  • Fast Scanning - Simultaneous 2-color scanning at 10 micron resolution, in about 8 minutes per slide for fast results.
  • Laser-Saver Feature - Programmable to turn on and off according to a given lab's workflow needs, thereby increasing the laser's lifespan while reducing instrument maintenance.
  • Instrument Start-up Diagnostics Software - Automatically assesses scanner performance parameters and provides researchers with a status report prior to scanning.
  • Web-Based Monitoring - Easily track the status of microarray through web-based scanning interface.
Bioanalyzer

Better productivity, reliability and speed through miniaturization and integration

Agilent Technologies is a leader in microfluidic Lab-on-a-Chip technology. This technology utilizes a network of channels and wells that are etched onto glass or polymer chips to build mini-laboratories. Pressure or electrokinetic forces move pico or nano liter volumes in finely controlled manner through the channels. Lab-on-a-Chip technology enables sample handling, mixing, dilution, electrophoresis and chromatographic separation, staining and detection on single integrated systems. The main advantages Lab-on-a-Chip are ease-of-use, speed of analysis, low sample and reagent consumption and high reproducibility due to standardization and automation.

Agilent's highly successful microfluidics-based platform for the analysis of DNA, RNA, proteins and cells. An excellent alternative to messy and labor-intensive gel electrophoresis techniques; delivering fast, reproducible, high quality digital data.