Real-Time PCR Applications

Real-Time PCR can be applied to traditional PCR applications as well as new applications that would have been less effective with traditional PCR. With the ability to collect data in the exponential growth phase, the power of PCR has been expanded into applications such as:

ABI 7900HT

• Viral Quantitation
• Quantitation of Gene Expression
• Array Verification
• Drug Therapy Efficacy
• DNA Damage measurement
• Quality Control and Assay Validation
• Pathogen detection
• Genotyping

Advantages of using Real-Time PCR

• Traditional PCR is measured at End-Point (plateau), while Real-Time PCR collects data in the exponential growth phase
• An increase in Reporter fluorescent signal is directly proportional to the number of amplicons generated
• The cleaved probe provides a permanent record amplification of an Amplicon
• Increase dynamic range of detection
• No-post PCR processing
• Detection is capable down to a 2-fold change

The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay