Agilent’s performance-leading microarray scanner just got better
The Agilent DNA microarray scanner is a 48-slide scanning system enabled by SureScan High-Resolution Technology is capable of scanning with 2, 3, 5 or 10 micron resolution that can read 1 x 3” glass slide microarrays and seamlessly extract them all using Agilent’s Feature Extraction Software with robust statistical analyses.
Reagents
Agilent's Low RNA Input Fluorescent Linear Amplification Kit, based on a simple, patented procedure, is designed to amplify and label either total RNA or poly A(+) RNA to generate micrograms of cRNA (antisense) or cDNA (sense).
SureHyb Chambers
Agilent's new microarray hybridization chambers and accessories have been specially designed to deliver more robust, more reproducible results enabling better detection of genes …in an easy to use, trusted format. The result is a solution to hybridization that leverages a unique mixing bubble to facilitate efficient mixing during the 17-hour incubation period to produce more meaningful microarray results.
Features
- Improved ease-of-use compared to the "legacy" 6-screw chamber-can now be assembled and disassembled without tools, in seconds rather than minutes
- Enhanced quality of data, delivered through reduced signal CV% and log ratio variability
- Higher sensitivity through increased feature signal strength with low background noise
- Reduced microarray variability (Microarray-to-microarray median CV = 14.5% legacy 6-screw model vs. 7.4% with the new G2534A model)
- Complete, economical solution based on Agilent's SureHyb platform (chamber assemblies, disposable gasket Slides, hybridization reagents)
Nanodrop-1000 Spectrophotometer
- Full-spectrum UV-Vis absorbance analyses (220-750nm)
- For measuring absorbance of DNA, RNA, dyes, proteins and microbial cell culture OD
- 1 ul of sample
- Large dynamic range: 2-3700 ng/ul of dsDNA
Analysis Gene Expression & CGH
The Agilent CGH Analytics software provides an intuitive user interface for visually exploring, detecting and analyzing aberration patterns from multiple Comparative Genomic Hybridization (CGH) microarray profiles. It accepts data output from Agilent Feature Extraction software and displays chromosomal deletions and amplifications at multiple zoom levels simultaneously. Take advantage of two different high speed statistical aberration detection algorithms to assist in the detection and mapping of aberration regions with high confidence.
60-mer microarrays
Agilent-designed 60-mer microarrays are processed through a powerful combination of probe design algorithms coupled with Agilent's probe selection process. This design method gives the customer maximum confidence in the probe design and prevents redundancy in gene coverage, empirically validated using 10 different tissues then, using Agilent's SurePrint technology, printed with a maskless & highly-flexible microarray printing based on industrial-scale inkjet technologies, and unique QC processes. The result is affordable, accessible pre-printed microarrays that content researchers can trust
Agilents Scanner
The Agilent DNA microarray scanner is a 48-slide scanning system that can read any mix of 1" x 3" glass slide microarrays (both Agilent and non-Agilent) and seamlessly feature extract them all using Agilent's Feature Extraction Software.
Built on a strong engineering foundation and years of experience in the microarray scanner market, the Agilent unleashes an enhanced-feature, powerful, fully-automated scanner with walk-away freedom.
Features
- Uniform Images & Enhanced Sensitivity - A component of Agilent's SureScan technology - Dynamic autofocus continually adjusts to keep features in focus while scanning. This minimizes the effects of gradients and aberrations associated with glass slides, and results in improved uniformity and enhanced sensitivity.
- Low Level Detection - Optimized precision optics, broad dynamic range and minimal spectral cross talk allow users to detect weak features and achieve rapid, accurate and dependable results.
- Fast Scanning - Simultaneous 2-color scanning at 10 micron resolution, in about 8 minutes per slide for fast results.
- Laser-Saver Feature - Programmable to turn on and off according to a given lab's workflow needs, thereby increasing the laser's lifespan while reducing instrument maintenance.
- Instrument Start-up Diagnostics Software - Automatically assesses scanner performance parameters and provides researchers with a status report prior to scanning.
- Web-Based Monitoring - Easily track the status of microarray through web-based scanning interface.
Bioanalyzer
Better productivity, reliability and speed through miniaturization and integration
Agilent Technologies is a leader in microfluidic Lab-on-a-Chip technology. This technology utilizes a network of channels and wells that are etched onto glass or polymer chips to build mini-laboratories. Pressure or electrokinetic forces move pico or nano liter volumes in finely controlled manner through the channels. Lab-on-a-Chip technology enables sample handling, mixing, dilution, electrophoresis and chromatographic separation, staining and detection on single integrated systems. The main advantages Lab-on-a-Chip are ease-of-use, speed of analysis, low sample and reagent consumption and high reproducibility due to standardization and automation.
Agilent's highly successful microfluidics-based platform for the analysis of DNA, RNA, proteins and cells. An excellent alternative to messy and labor-intensive gel electrophoresis techniques; delivering fast, reproducible, high quality digital data.
MOgene, LC is now a Service Provider for all of the Nimblegen Products
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Gene Expression
Roche NimbleGen provides a unique combination of high-density arrays, long-oligo probes, and flexible design capability that together provide superior results for advanced gene expression analysis. NimbleGen microarrays enable accurate, sensitive, and specific interrogation of genome-wide expression for any sequenced and annotated genome. Researchers also can tile through a genome at any desired spacing to discover genome-wide expression activity in both coding and noncoding regions and map transcripts with unparalleled accuracy. NimbleGen microarrays and services for both prokaryotic and eukaryotic genomes are the gene expression platform of choice as evidenced by a growing list of scientific publications.
CGH
Comparative Genomic Hybridization (CGH) measures DNA copy number differences between a test and reference genome. NimbleGen offers two types of CGH array products: whole-genome and custom targeted arrays. Whole-genome arrays measure DNA copy number gains and losses across entire genomes, while custom targeted arrays can interrogate a single chromosomal region or multiple loci of interest. In addition, custom designs can be created with either uniform or mixed-density probe spacing.
ChIP-on-Chip
Roche NimbleGen provides the most sensitive ChIP-chip (chromatin immunoprecipitation on chip) microarrays and services on the market. These arrays enable rapid identification of precise binding sites of specific DNA-binding proteins—such as transcription factors, histones, and polymerases—within a target genome, as well as uncover chromatin remodeling in any region of a genome. NimbleGen ChIP-chip microarrays and services have become the platform of choice supported by an ever-growing list of peer-reviewed publications.
DNA Methylation
Roche NimbleGen provides the most sensitive and specific DNA methylation microarrays and services on the market. Affinity-based methods for enrichment of methylated DNA fragments, such as MeDIP (Methylated DNA immunoprecipitation) or the MBD (Methyl Binding Domain Protein) methods, are the preferred sample preparation methods for identifying methylated DNA on NimbleGen arrays. Coupled with one of these enrichment methods, our arrays and services offer:
- Precise identification of methylated DNA regions across whole genomes or within biologically focused regions including promoters and CpG islands.
- Highly sensitive detection of methylated CpGs due to use of long, isothermal (50-75mer) oligonucleotide probes.
- Comprehensive catalog collection of whole-genome tiling arrays and targeted Deluxe Promoter arrays for multiple organisms.
NextGen Sequencing services using Roche's 454 GS FLX using Titanium chemistry
The Genome Sequencer FLX Instrument, powered by GS FLX Titanium and Standard series reagents, features a groundbreaking combination of long reads, exceptional accuracy and high throughput. The breadth of applications, including de novo sequencing, resequencing of whole genomes and target DNA regions, metagenomics and RNA analysis have resulted in hundreds of peer-reviewed publications to date.
System Benefits
- Obtain more comprehensive data.
Generate more than 1,000,000 individual reads with improved Q20 read length of 400 bases per 10-hour instrument run
- Expand your project capabilities.
Harness the power of DNA sequencing for your complete genome project with one versatile system. Combine long single reads and Long-Tag Paired end reads to completely assemble genomes-often within a single run.
- Reduce your cost per result.
Ultra-high throughput delivers the data you need at an affordable price while longer reads reduce over-sampling requirements, keeping data requirements manageable. Benefit from flexible sample-loading options that support various throughput and experimental design needs, enabling efficient use of reagents and consumables.
- Increase your productivity.
Streamline library preparation of genomics samples and eliminate the laborious tasks of cloning and colony picking - genomic libraries are constructed in hours in a single tube.
- Driving results, not the accumulation of data files.
Perform data analysis without the need for enterprise scale IT solutions with the included easy-to-use software tools - GS De Novo Assembler, GS Reference Mapper, and GS Amplicon Variant Analyzer*. Straightforward interpretation of data means faster discovery of biologically meaningful results.
Real-Time PCR Applications
Real-Time PCR can be applied to traditional PCR applications as well as new applications that would have been less effective with traditional PCR. With the ability to collect data in the exponential growth phase, the power of PCR has been expanded into applications such as:
ABI 7900HT
- Viral Quantitation
- Quantitation of Gene Expression
- Array Verification
- Drug Therapy Efficacy
- DNA Damage measurement
- Quality Control and Assay Validation
- Pathogen detection
- Genotyping
Advantages of using Real-Time PCR
- Traditional PCR is measured at End-Point (plateau), while Real-Time PCR collects data in the exponential growth phase
- An increase in Reporter fluorescent signal is directly proportional to the number of amplicons generated
- The cleaved probe provides a permanent record amplification of an Amplicon
- Increase dynamic range of detection
- No-post PCR processing
- Detection is capable down to a 2-fold change
The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay
NuGEN has revolutionized amplification and labeling technologies that enable life science researchers to mine genomic samples from extremely small, degraded, and hard-to-replace specimens.
As a partner in the discovery process, NuGEN brings solutions to top global pharmaceutical companies, academic institutions, and cutting edge biotechnology companies to meet their toughest challenges in clinical research, expression profiling, and biomarker and signature discovery.
NuGEN’s proprietary SPIA® technology is an elegant method for linear isothermal amplification of nucleic acids. This simple, high-fidelity approach to amplification enables global genomic analysis of limited and/or compromised biological samples, overcoming the challenges of sample quality and quantity with a high degree of sensitivity and accuracy.
Complex microarray platforms and various dissection technologies have evolved to enable analysis of highly homogenous biological samples. As a result, researchers have gained new opportunities to work with samples comprised of low numbers of cells such as laser captured microdissections, fine-needle aspirates, sorted cells, circulating tumor cells, embryonic structures, biopsies and Formalin-Fixed Paraffin-Embedded (FFPE) samples. Such sources often yield extremely small amounts of compromised RNA or DNA, adding to the difficulty in investigating these biological samples.
These challenges are met with NuGEN’s innovative technologies, which are at the core of a robust, sensitive, and easy to automate family of products and solutions that enable today’s discovery and clinical research projects
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