Sequence Capture

NimbleGen Sequence Capture arrays enable you to produce targeted, sequencing-ready samples in your lab for use with the Genome Sequencer FLX System and GS FLX Titanium Kits from 454 Life Sciences. Utilizing high-density, long-oligo NimbleGen arrays, either the human exome or the human genomic regions you specify are hybridized and eluted, ready for subsequent amplification and sequencing. This array-based process offers significant speed and scalability advantages over current PCR-based methods for targeted enrichment.

To supply our customers with an affordable, high-quality solution, we have validated, and optimized protocols for obtaining enriched DNA that can be directly and easily integrated into the workflow of the 454 Genome Sequencer FLX instrument. The 454 Genome Sequencer FLX instrument with FLX Titanium Kits delivers read lengths of 400bp (500MB raw sequence per PTP) and is the most appropriate sequencing technology for the NimbleGen Sequence Capture solution.

MOgene also provides 454 sequencing services

• Captured samples can be fed directly into the 454 Titanium Shot-gun library preparation protocol for addition of sequencing adapters.
• Sequence your captured samples on the 454 GS FLX to generate high-confidence sequencing data using up to ~1,000,000 reads for roughly 500Mb of raw sequence data per picotiter plate.
• 400bp reads enable haplotyping of 400bp and identification of insertions/deletions from single to tens of base pairs.