454 Services

Sequence Capture/Targeted Resequencing

Overview

NimbleGen's Sequence Capture arrays can capture up to 30Mb (2.1M) or 5Mb (385K) genomic regions as enriched DNA fragments and can be sequenced to the researchers desired depth and % base coverage using the GS FLX Titanium series reagents. In addition, the 2.1M Human Exome arrays are capable of capturing ~180,000 human coding exons and ~550 miRNA exons (from the CCDS database, build April 30th, 2008) on a single array. Benefit from GS FLX Titanium long reads for haplotyping and straightforward identification of insertions and deletions, without GC bias.

Publications

• Albert et al. Direct selection of human genomic loci by microarray hybridization. Nature Methods 4: 903-5. November 2007.
• Yeager et al. Comprehensive resequence analysis of a 136 kb region of human chromosome 8q24 associated with prostate and colon cancers. Human Genetics. ePub August 14 2008.

Click here to see all Sequence Capture/Targeted Region publications.

Data Analysis tools:

GS Mapper Software

How It Works

• The genomic DNA sample is fragmented and polished
• The linkers are ligated to the fragments
• The sample is hybridized to a NimbleGen Sequence Capture array
• The unbound fragments are washed away
• The target fragments are eluted using the NimbleGen Elution System
• The target enriched pool is amplified
• The enriched, amplified pool is then verified by qPCR
• The enriched sample is then ready for high-throughput sequencing with the Genome Sequencer FLX by 454 Life Sciences