454 Services

Prokaryote Whole Genome

Overview

The Genome Sequencer FLX System facilitates the completion of near-finished draft sequences of multiple whole prokaryote genomes on a single 10-hour instrument run. Assembly of long shotgun reads (400 base pairs) yields minimal contigs and the addition of multi-span paired end reads (>150 base tags from each end of a 3 Kb, 8 Kb or 20 Kb span) orders contigs into as few as one scaffold. For some microbial genomes, use only one 8 Kb span paired end library to assemble genomes into a single scaffold per chromosome, without the addition of shotgun reads. The sequencing depth achieved with 454 Sequencing systems ensures accurate characterization of microbial or bacterial diversity, sensitive detection of even rare mutations, and rapid discovery of disease causing agents.