HIV Sequencing
Overview
Characterize and quantify the viral population within an infected individual with Ultra-Deep 454 Sequencing. The Genome Sequencer FLX System enables accurate and quantitative assessment of HIV quasi-species through the sequencing of target genes (ex. protease and reverse transcriptase genes). Sequencing hundreds of thousands of individual DNA molecules simultaneously, the GS FLX System detects rare genetic variants constituting as little as 1% of the population, levels far deeper than those of traditional sequencing methods. Furthermore, long reads elucidate haplotypes within each amplicon.
Data Analysis tools:
GS Amplicon Variant Analyzer Software
How it works:
Ultra Deep 454 Sequencing
Targeted amplicon sequencing on amplicons derived from cDNA fragments, amplified with lucus specific primers covering the major variant positions in desired genes.
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